Molecular biology methods for blood cell antigen genotyping in reference laboratories

Methods in Molecular Biology

For almost 30 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readilyreproducible step-by-step fashion, opening with a...

Grand Challenges in Molecular Antigen-Presenting Cell-Biology

All adaptive immune responses are initiated by dedicated antigen-presenting cells (APCs), mainly dendritic cells (DC), whose principal purpose is the activation of T-cells. To this end, APCs need to correctly perform a series of complex functions: First, they need to home to tissues to be available for collecting antigens. Then they need to transport the antigen to secondary lymphatic organs, w...

Formal Methods in Molecular Biology

This report documents the program and the outcomes of the Seminar 11151 ‘Formal Methods in Molecular Biology’ that took place in Dagstuhl, Germany, on 10.–15. April, 2011. The most recent advances in Systems Biology were discussed, as well as and the contribution of computational formalisms to the modeling of biological systems, with the focus on stochasticity. About 30 talks were given. The pa...

Methods in Cell Biology

I. Why Tubulin from Nonneural Sources?II. General Principles of Tubulin PurificationIII. Specific Properties of Distinct Nonneural SourcesA. Metazoan Sources (e.g., Tissue Culture Cells, Chicken Red Blood Cells)B. Tetrahymena thermophilaC. Kinetoplastid Parasites (Leishmania, Trypanosoma)D. Saccharomyces cerevisiaeE. Land PlantsIV. Genetic Manipulation of Tub...

Methods in Cell Biology